Journal: Experimental and Therapeutic Medicine

Article Title: TRIP6 regulates the proliferation, migration, invasion and apoptosis of osteosarcoma cells by activating the NF-κB signaling pathway

doi: 10.3892/etm.2020.8466

Figure Lengend Snippet: TRIP6 plays a role in Os cell proliferation. (A) Western blot analysis of TRIP6 protein expression level in TRIP6 silenced-U2OS cell lines and (B) quantification. (C) Western blot analysis of TRIP6 protein expression level in TRIP6 transduced-MG63 cell lines and (D) quantification. Data were normalized to GAPDH and are presented as the mean ± SD from three independent experiments. MTT assay results from (E) U2OS cells and (F) MG63 cells indicated that growth rates increased in TRIP6-overexpressing cells and decreased in TRIP6-silenced cells. (G) U2OS cells colony formation assay (H) results and (I) MG63 cells colony formation assay (J) results indicated that growth rates increased in TRIP6-overexpressing cells and decreased in TRIP6-silenced cells. Colony formation, classified as colonies >0.1 mm diameter, was quantified after 12 days of culture. Apoptosis of U2-OS and MG63 cells analyzed by flow cytometry after different treatments. (K) The apoptotic rate was decreased in U2OS-NC cells (6.42%) (L) compared with U2OS-TRIP6-siRNA cells (15.61%). (M) The apoptosis rates of (K) and (L) were statistically significant. (N) The apoptotic rate was 7.16% in the MG63-Vector group and (O) 3.48% in the MG63-TRIP6-flag group. (P) The apoptosis rates of (N) and (O) were statistically significant. *P<0.05 vs. control group. Os, osteosarcoma; TRIP6, thyroid hormone receptor-interacting protein 6; NC, negative control; siRNA, small interfering RNA.

Article Snippet: After cells were harvested, washed twice with PBS and resuspended in 450 μl binding buffer (Annexin V-FITC cell apoptosis assay kit; Beyotime Institute of Biotechnology), Annexin V-FITC was added (5 μl/well).

Techniques: Western Blot, Expressing, MTT Assay, Colony Assay, Flow Cytometry, Plasmid Preparation, Control, Negative Control, Small Interfering RNA

Journal: International Journal of Oncology

Article Title: Eupalinolide A induces autophagy via the ROS/ERK signaling pathway in hepatocellular carcinoma cells in vitro and in vivo

doi: 10.3892/ijo.2022.5421

Figure Lengend Snippet: Effect of EA on apoptosis and necroptosis of human hepatocellular carcinoma cells. (A and B) Western blot analysis of the expression levels of apoptosis and necroptosis-related proteins at 48 h in MHCC97-L and HCCLM3 cells. β-actin was used as a control. (C and D) Effect of EA on apoptosis in MHCC97-L and HCCLM3 cells, as determined by flow cytometry. n=5. (E) Viability of MHCC97-L and HCCLM3 cells treated with or without apoptosis or necroptosis inhibitors was measured by Cell Counting Kit 8 assays. n=6. All data are shown as the mean ± SD. *** P<0.001. EA, eupalinolide A; C-, cleaved; p-, phosphorylated.

Article Snippet: The cells were then collected, washed with cold PBS and suspended in 200 μ l binding buffer (Annexin V-FITC/PI apoptosis assay kit; Neobioscience Technology Co., Ltd.) at a density of 5×10 5 /ml.

Techniques: Western Blot, Expressing, Control, Flow Cytometry, Cell Counting